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coli synthetic auxotrophs that rely solely on externally provided 4-azido-phenylalanine (AzF) for growth by introducing in-frame amber codons into the essential genes ( dnaN , adk , tyrS , and pgsA ), and these synthetic auxotrophic cells were further demonstrated to exhibit robust AzF-dependent growth in living mice

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the mechanism study that removed the beta-3 adrenergic receptor in mice and found the fat burning effect of AOD-9604 disappeared completely, confirming exactly which receptor the peptide depends on